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KMID : 0380020030180010065
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Korean Journal of Biotechnology and Bioengineering
2003 Volume.18 No. 1 p.65 ~ p.69
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Chromatographic Characterization of Pentoxitylline and a Mixture of Caffeine-theophylline Imprinted Poiymers
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Hong Seung-Pyo
Wang Duk-Sun
Koo Yoon-Mo
Row Kyung-Ho
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Abstract
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Two molecular imprinted polymers (MIPs) were prepared by using pentoxifylline and a mixture of caffeine-theophylline (2:1, mole ratio) as the templates, MAA as the monomer, and EDMA as the crosslinker The obtained polymer particles (particle size 25-35 ) were packed into a HPLC column (1503.9mm). The selectivity and chromatographic characterization of the imprinted polymers were studied using pentoxifylline, caffeine, theophylline, theobromine as samples and acetonitrile as the mobile phase, Both imprinted polymers showed increased affinity for structural analogues. By using a mixture of caffeine-theophylline templates MIPs, good separations of caffeine, theobromine and theophylline were obtained, and pentoxifylline and caffeine were partly separated.
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KEYWORD
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Molecular imprinting, molecular recognition, hydrogen bond, HPLC
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